ABSTRACT
Objective To study the effect of GLP-1 on the liver function and the signal pathway of TLR4/NF-κB in NAFLD rats.Methods Forty-five rats were randomly divided into two groups,15 with normal diet fed and 30 with high-fat diet,so as to form NAFLD model.In the tenth week,28 rats fed with high-fat diet were randomly divided into two groups,the model control group (14) and the GLP-1 intervention group (14 rats).The normal diet fed in 15 rats served as the normal control group.After the model group and the control group rats were injected with saline treatment,the intervention group rats were injected with liraglutide treatment.We observed the changes in the activity of rats,body weight,appetite,urine and other conditions.Results Compared with the control group,the liver index,liver mass,ALT,AST,TG and TC of the model rats were significantly higher than those of the control group (P ＜ 0.05).The liver index,liver mass,ALT,AST,TG and TC of the model rats were significant lower in the GLP-1 intervention group than those in the model control group (P ＜ 0.05).Compared with the normal control group,the expression of TLR4 and NF-κB protein in the model control group increased in the model control group (P ＜ 0.05).Compared with the model control group,the expression of TLR4 and NF-κB protein was decreased in the GLP-1 intervention group (P ＜ 0.05).Conclusion GLP-1 can significantly reverse the disorder of glucose and lipid metabolism and impaired liver function in NAFLD rats,and GLP-1 can significantly reduce the expression of NF-κB and TLR4 protein in the liver tissues of rats.
ABSTRACT
Objective To research the obesity-related gene (FTO) and forkhead transcription factors O1 (FoxO1) protein expression level in the livers of non-alcoholic fatty liver disease(NAFLD) rat model. Methods The animal model of NAFLD in rats was prepared by feeding high energy and high fat feed. Then the rat blood and liver tissue were collected for detecting the liver index and blood biochemical indexes, including triglycerides (TG), total cholesterol (TC), alanine aminotransferase (AST), aspartate aminotransferase(ALT) ,alkaline phosphatase(ALP),high-density lipoprotein (HDL) and low density lipoprotein(LDL) ;the liver pathological examination was performed;FTO protein and Fox O1 protein expression levels in rat liver were detected by using the immunohistochemical assay. Results The rat liver weight,body weight and liver index after 8 weeks in the model group were higher than those in the control group,the difference was statistically significant (P<0.05);the levels of AST, ALT, LDL, ALP, TG and TC in the model group were higher than those in the control group,the difference was statistically significant (P<0. 05),the HDL level in the model group was lower than that in the control group, the difference was statistically significant (P<0.05) ;the model group produced steatosis and inflammation in hepatic lobule part,while the control group had no these lesions;the FTO prot ein and FoxO1 protein expression levels in liver of the model group were higher than those in the control group,the difference was statistically significant (P<0.05). Conclusion FTO and FoxO1 interaction may disturb the normal energy and fat metabolism.